Project
ENCODE: H3K4me3 ChIP-Seq of primary human cells
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CREMA is a free online tool that recognizes most important transcription factors that change the chromatin state across different samples.

Results for HINFP1

Z-value: 0.56

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Transcription factors associated with HINFP1

Gene Symbol Gene ID Gene Info

Activity of the HINFP1 motif across conditions

Conditions sorted by the z-value of the HINFP1 motif activity

Move your cursor over a bar to see sample name and corresponding Z-value.

Top target CREs of the motif:

Cis Regulatory Element (CRE) Target Score Top associated gene Gene Info Distance of CRE to TSS CRE/Gene association probability
chr2_30454378_30455881 0.45 LBH
 limb bud and heart development
 83
 0.98
chr20_3994903_3996170 0.42 RNF24
 ring finger protein 24
 500
 0.83
chr17_57408824_57410302 0.40 YPEL2
 yippee-like 2 (Drosophila)
 500
 0.78
chr1_62784626_62784933 0.38 KANK4
 KN motif and ankyrin repeat domains 4
 193
 0.95
chr2_96809842_96811219 0.38 DUSP2
 dual specificity phosphatase 2
 649
 0.65
chr3_96531977_96533205 0.34 EPHA6
 EPH receptor A6
 834
 0.76
chr7_26415747_26416790 0.34 AC004540.4
53
 0.98
chr1_169454193_169456202 0.32 SLC19A2
 solute carrier family 19 (thiamine transporter), member 2
 28
 0.98
chr16_67197260_67198336 0.31 HSF4
 heat shock transcription factor 4
 510
 0.52
chr1_231297648_231298418 0.26 TRIM67
 tripartite motif containing 67
 175
 0.95
chr20_708643_709189 0.24 SLC52A3
 solute carrier family 52 (riboflavin transporter), member 3
 40215
 0.13
chr19_2251617_2252237 0.23 ENSG00000267021
 .
 1289
 0.21
chr1_11724348_11725085 0.21 FBXO6
 F-box protein 6
 535
 0.67
chr18_47017567_47018883 0.21 RPL17
 ribosomal protein L17
 15
 0.51
chr20_35974122_35974616 0.20 SRC
 v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
 188
 0.96
chr17_79875057_79876036 0.20 SIRT7
 sirtuin 7
 524
 0.46
chr10_62492315_62493446 0.20 ANK3
 ankyrin 3, node of Ranvier (ankyrin G)
 368
 0.92
chr11_120894558_120895785 0.19 TBCEL
 tubulin folding cofactor E-like
 341
 0.93
chr13_22032188_22033345 0.18 ZDHHC20
 zinc finger, DHHC-type containing 20
 648
 0.76
chr22_24989005_24989426 0.18 FAM211B
 family with sequence similarity 211, member B
 185
 0.9
chr3_196695819_196696888 0.17 PIGZ
 phosphatidylinositol glycan anchor biosynthesis, class Z
 611
 0.66
chr8_69242814_69243988 0.17 C8orf34
 chromosome 8 open reading frame 34
 56
 0.82
chr7_101460813_101461304 0.17 CUX1
 cut-like homeobox 1
 138
 0.97
chr6_163833862_163834944 0.17 QKI
 QKI, KH domain containing, RNA binding
 1272
 0.63
chr13_20766378_20767168 0.16 GJB2
 gap junction protein, beta 2, 26kDa
 264
 0.91
chr12_90101448_90102652 0.15 ATP2B1
 ATPase, Ca++ transporting, plasma membrane 1
 558
 0.78
chr3_160822448_160823114 0.15 B3GALNT1
 beta-1,3-N-acetylgalactosaminyltransferase 1 (globoside blood group)
 43
 0.67
chr4_187025490_187026623 0.15 FAM149A
 family with sequence similarity 149, member A
 175
 0.94
chr18_12308279_12308732 0.15 TUBB6
 tubulin, beta 6 class V
 243
 0.92
chr1_935525_936213 0.15 HES4
 hes family bHLH transcription factor 4
 317
 0.77
chr1_236958581_236959780 0.14 MTR
 5-methyltetrahydrofolate-homocysteine methyltransferase
 198
 0.96
chr10_123733982_123734880 0.14 NSMCE4A
 non-SMC element 4 homolog A (S. cerevisiae)
 158
 0.96
chr2_71693301_71694189 0.13 DYSF
 dysferlin
 87
 0.98
chr9_86595749_86596582 0.13 RMI1
 RecQ mediated genome instability 1
 452
 0.51
chr16_84733607_84734797 0.13 USP10
 ubiquitin specific peptidase 10
 556
 0.79
chr12_95942120_95942537 0.13 USP44
 ubiquitin specific peptidase 44
 246
 0.94
chr9_131418722_131419532 0.13 WDR34
 WD repeat domain 34
 61
 0.96
chr7_111201649_111202512 0.13 IMMP2L
 IMP2 inner mitochondrial membrane peptidase-like (S. cerevisiae)
 100
 0.98
chr19_13056666_13057332 0.13 RAD23A
 RAD23 homolog A (S. cerevisiae)
 293
 0.71
chr20_1875023_1875335 0.12 SIRPA
 signal-regulatory protein alpha
 25
 0.98
chrX_70150677_70150828 0.12 SLC7A3
 solute carrier family 7 (cationic amino acid transporter, y+ system), member 3
 194
 0.95
chr6_100061682_100062402 0.12 PRDM13
 PR domain containing 13
 7436
 0.19
chr10_50606076_50606780 0.11 DRGX
 dorsal root ganglia homeobox
 2931
 0.27
chr6_1605443_1605730 0.11 FOXC1
 forkhead box C1
 5095
 0.33
chr9_37034211_37034515 0.11 PAX5
 paired box 5
 260
 0.93
chr13_67804057_67805835 0.11 PCDH9
 protocadherin 9
 478
 0.9
chr1_110753494_110754182 0.11 KCNC4
 potassium voltage-gated channel, Shaw-related subfamily, member 4
 257
 0.84
chr16_30216869_30217924 0.11 RP11-347C12.3
 Uncharacterized protein
 31
 0.94
chr20_57465496_57466112 0.10 GNAS
 GNAS complex locus
 553
 0.66
chr18_3262315_3263346 0.10 MYL12B
 myosin, light chain 12B, regulatory
 124
 0.92
chr2_129079627_129080191 0.10 HS6ST1
 heparan sulfate 6-O-sulfotransferase 1
 3758
 0.29
chr10_94822298_94822949 0.10 CYP26C1
 cytochrome P450, family 26, subfamily C, polypeptide 1
 1602
 0.34
chr7_149156320_149157211 0.10 ZNF777
 zinc finger protein 777
 1449
 0.45
chr16_718193_719247 0.09 RHOT2
 ras homolog family member T2
 511
 0.45
chr5_131745958_131747299 0.09 C5orf56
 chromosome 5 open reading frame 56
 45
 0.96
chr3_12910219_12910517 0.09 AC034198.7
18210
 0.14
chr8_37620085_37620952 0.08 PROSC
 proline synthetase co-transcribed homolog (bacterial)
 400
 0.79
chr6_108497513_108497836 0.07 NR2E1
 nuclear receptor subfamily 2, group E, member 1
 7707
 0.18
chr2_71453255_71454205 0.07 PAIP2B
 poly(A) binding protein interacting protein 2B
 483
 0.83
chr6_168720263_168720414 0.07 DACT2
 dishevelled-binding antagonist of beta-catenin 2
 52
 0.98
chr17_27892819_27893926 0.07 ABHD15
 abhydrolase domain containing 15
 783
 0.31
chr7_139875207_139876022 0.07 KDM7A
 lysine (K)-specific demethylase 7A
 1127
 0.39
chr17_40202025_40202687 0.07 CTD-2132N18.2
7221
 0.11
chr7_100081068_100081412 0.07 ENSG00000266372
 .
 226
 0.55
chr7_92218956_92219793 0.07 FAM133B
 family with sequence similarity 133, member B
 10
 0.98
chr6_30457259_30457905 0.07 HLA-E
 major histocompatibility complex, class I, E
 338
 0.84
chr6_45631346_45631892 0.06 ENSG00000252738
 .
 17778
 0.29
chr10_112403107_112403970 0.06 RBM20
 RNA binding motif protein 20
 617
 0.55
chr20_3827532_3828225 0.06 MAVS
 mitochondrial antiviral signaling protein
 388
 0.8
chr1_27687344_27687533 0.06 MAP3K6
 mitogen-activated protein kinase kinase kinase 6
 3004
 0.16
chr10_21823798_21823949 0.06 MLLT10
 myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 10
 195
 0.92
chr19_4374282_4374693 0.06 SH3GL1
 SH3-domain GRB2-like 1
 5802
 0.08
chr7_132260669_132261651 0.05 PLXNA4
 plexin A4
 69
 0.98
chr4_186130212_186131033 0.05 KIAA1430
 KIAA1430
 36
 0.92
chr22_20306851_20307537 0.05 DGCR6L
 DiGeorge syndrome critical region gene 6-like
 338
 0.74
chr14_56585152_56585591 0.05 PELI2
 pellino E3 ubiquitin protein ligase family member 2
 278
 0.94
chr8_143533593_143534231 0.05 BAI1
 brain-specific angiogenesis inhibitor 1
 3121
 0.27
chr2_178128520_178129352 0.05 AC079305.10
151
 0.76
chr18_29264680_29264911 0.05 B4GALT6
 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 6
 109
 0.91
chr15_66585571_66586039 0.05 DIS3L
 DIS3 mitotic control homolog (S. cerevisiae)-like
 80
 0.53
chr11_70962576_70962972 0.05 SHANK2
 SH3 and multiple ankyrin repeat domains 2
 849
 0.72
chr4_87812935_87814058 0.05 C4orf36
 chromosome 4 open reading frame 36
 52
 0.98
chr6_29894399_29894823 0.05 HLA-A
 major histocompatibility complex, class I, A
 14426
 0.18
chr6_11043780_11044551 0.05 ELOVL2-AS1
 ELOVL2 antisense RNA 1
 172
 0.73
chr1_156829720_156830506 0.05 NTRK1
 neurotrophic tyrosine kinase, receptor, type 1
 494
 0.64
chr17_11144173_11144324 0.05 SHISA6
 shisa family member 6
 332
 0.91
chr1_233463556_233464041 0.05 MLK4
 Mitogen-activated protein kinase kinase kinase MLK4
 284
 0.94
chr18_11981851_11982483 0.04 IMPA2
 inositol(myo)-1(or 4)-monophosphatase 2
 498
 0.77
chrY_21154472_21155007 0.04 ENSG00000252766
 .
 26234
 0.25
chr2_136289143_136290038 0.04 R3HDM1
 R3H domain containing 1
 503
 0.62
chr20_52209104_52210193 0.04 ZNF217
 zinc finger protein 217
 730
 0.68
chr17_77817761_77818338 0.04 CBX4
 chromobox homolog 4
 4821
 0.18
chr17_16487582_16488116 0.04 ZNF287
 zinc finger protein 287
 15329
 0.16
chr1_207082522_207083417 0.04 IL24
 interleukin 24
 11791
 0.14
chr6_84569350_84570131 0.03 CYB5R4
 cytochrome b5 reductase 4
 365
 0.9
chr19_50354346_50354967 0.03 PTOV1
 prostate tumor overexpressed 1
 174
 0.58
chr17_40829888_40830324 0.03 PLEKHH3
 pleckstrin homology domain containing, family H (with MyTH4 domain) member 3
 1058
 0.27
chr1_29448339_29449190 0.03 TMEM200B
 transmembrane protein 200B
 249
 0.93
chr11_450302_450786 0.03 PTDSS2
 phosphatidylserine synthase 2
 264
 0.82
chr7_107220684_107221346 0.03 BCAP29
 B-cell receptor-associated protein 29
 102
 0.86
chr7_96635926_96636202 0.03 DLX6
 distal-less homeobox 6
 673
 0.48
chr3_14644257_14644427 0.03 AC090952.5
46597
 0.13
chr4_109093413_109093782 0.03 ENSG00000232021
 .
 258
 0.93
chr16_58059170_58059939 0.03 MMP15
 matrix metallopeptidase 15 (membrane-inserted)
 84
 0.96
chr2_33171552_33172146 0.03 LTBP1
 latent transforming growth factor beta binding protein 1
 190
 0.93
chr3_182896879_182897541 0.03 LAMP3
 lysosomal-associated membrane protein 3
 15583
 0.21
chrX_49033425_49033576 0.03 PLP2
 proteolipid protein 2 (colonic epithelium-enriched)
 5227
 0.09
chr18_32956679_32957498 0.03 ZNF396
 zinc finger protein 396
 186
 0.96
chr2_232526625_232527001 0.03 ENSG00000239202
 .
 15829
 0.16
chr20_32077934_32078551 0.02 CBFA2T2
 core-binding factor, runt domain, alpha subunit 2; translocated to, 2
 319
 0.89
chr2_234263629_234264304 0.02 DGKD
 diacylglycerol kinase, delta 130kDa
 812
 0.51
chr12_58129779_58130334 0.02 AGAP2
 ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
 1563
 0.16
chr6_159360525_159360963 0.02 ENSG00000223191
 .
 13141
 0.19
chr10_8091983_8093018 0.02 GATA3
 GATA binding protein 3
 4156
 0.36
chr5_159546809_159547203 0.02 PWWP2A
 PWWP domain containing 2A
 576
 0.75
chr3_52016673_52017699 0.02 ABHD14B
 abhydrolase domain containing 14B
 239
 0.49
chr17_17603690_17604249 0.02 RAI1
 retinoic acid induced 1
 18160
 0.17
chr5_174871055_174871780 0.02 DRD1
 dopamine receptor D1
 206
 0.95
chr5_92906290_92906587 0.02 ENSG00000237187
 .
 61
 0.98
chr12_133532729_133533161 0.02 ZNF605
 zinc finger protein 605
 53
 0.49
chr1_10458416_10458839 0.02 PGD
 phosphogluconate dehydrogenase
 22
 0.96
chr3_139654401_139654885 0.02 CLSTN2
 calsyntenin 2
 616
 0.84
chr11_805194_805716 0.02 PIDD
 p53-induced death domain protein
 210
 0.8
chr19_12780320_12781072 0.02 WDR83
 WD repeat domain 83
 179
 0.62
chr5_139028499_139029117 0.02 CXXC5
 CXXC finger protein 5
 290
 0.92
chr2_237087502_237088103 0.02 GBX2
 gastrulation brain homeobox 2
 10790
 0.15
chr9_139439215_139440028 0.02 NOTCH1
 notch 1
 693
 0.35
chr13_21635697_21635989 0.02 LATS2
 large tumor suppressor kinase 2
 157
 0.95
chr12_109747933_109748276 0.02 FOXN4
 forkhead box N4
 1079
 0.55
chr7_32535106_32535501 0.02 AVL9
 AVL9 homolog (S. cerevisiase)
 19
 0.88
chr1_224622519_224623397 0.02 WDR26
 WD repeat domain 26
 957
 0.59
chr12_27932510_27932994 0.02 KLHL42
 kelch-like family member 42
 201
 0.87
chr6_24720330_24720995 0.02 C6orf62
 chromosome 6 open reading frame 62
 42
 0.97
chr13_28528843_28528994 0.02 ATP5EP2
 ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit pseudogene 2
 9575
 0.14
chr20_34189521_34189992 0.02 SPAG4
 sperm associated antigen 4
 14058
 0.11
chr7_75911448_75912023 0.01 SRRM3
 serine/arginine repetitive matrix 3
 197
 0.93
chr14_45366753_45367297 0.01 C14orf28
 chromosome 14 open reading frame 28
 487
 0.59
chr1_148176495_148177244 0.01 PPIAL4D
 peptidylprolyl isomerase A (cyclophilin A)-like 4D
 25667
 0.21
chr5_6714492_6714756 0.01 PAPD7
 PAP associated domain containing 7
 94
 0.98
chr11_67275372_67275646 0.01 CDK2AP2
 cyclin-dependent kinase 2 associated protein 2
 53
 0.93
chr12_19283254_19284293 0.01 PLEKHA5
 pleckstrin homology domain containing, family A member 5
 1043
 0.58
chr5_129239637_129240094 0.01 CHSY3
 chondroitin sulfate synthase 3
 300
 0.88
chr11_64901308_64901962 0.01 SYVN1
 synovial apoptosis inhibitor 1, synoviolin
 265
 0.76
chr16_75033808_75034560 0.01 ZNRF1
 zinc and ring finger 1, E3 ubiquitin protein ligase
 941
 0.6
chr7_27179265_27179416 0.01 HOXA3
 homeobox A3
 485
 0.41
chrX_129299242_129299778 0.01 AIFM1
 apoptosis-inducing factor, mitochondrion-associated, 1
 128
 0.96
chr19_6767133_6767491 0.01 SH2D3A
 SH2 domain containing 3A
 134
 0.93
chr6_18277489_18278102 0.01 ENSG00000199715
 .
 4298
 0.21
chr12_6833255_6834039 0.01 COPS7A
 COP9 signalosome subunit 7A
 175
 0.85
chr4_190936109_190936296 0.01 ENSG00000199572
 .
 91
 0.95
chr1_24829331_24829634 0.01 RCAN3
 RCAN family member 3
 95
 0.97
chr7_157072082_157072490 0.01 ENSG00000266453
 .
 26201
 0.21
chr17_46674549_46675551 0.01 HOXB-AS3
 HOXB cluster antisense RNA 3
 1249
 0.2
chr2_220416827_220417484 0.01 ENSG00000265252
 .
 3286
 0.09
chr17_56234195_56234709 0.01 OR4D1
 olfactory receptor, family 4, subfamily D, member 1
 1958
 0.26
chr10_119135118_119135768 0.01 PDZD8
 PDZ domain containing 8
 465
 0.84
chr11_68228271_68228585 0.01 PPP6R3
 protein phosphatase 6, regulatory subunit 3
 147
 0.96
chr17_12920409_12921278 0.01 ELAC2
 elaC ribonuclease Z 2
 64
 0.97
chr11_119454980_119455308 0.01 RP11-196E1.3
23991
 0.19
chr17_38296734_38297043 0.01 CASC3
 cancer susceptibility candidate 3
 207
 0.9
chr5_5421590_5422497 0.01 KIAA0947
 KIAA0947
 764
 0.8
chr3_141120790_141121159 0.01 ZBTB38
 zinc finger and BTB domain containing 38
 217
 0.95
chr1_165796835_165797193 0.00 TMCO1
 transmembrane and coiled-coil domains 1
 22
 0.51
chr19_4304444_4304595 0.00 FSD1
 fibronectin type III and SPRY domain containing 1
 78
 0.93
chr7_74306205_74306628 0.00 STAG3L2
 stromal antigen 3-like 2 (pseudogene)
 259
 0.91
chr19_16999844_17000017 0.00 F2RL3
 coagulation factor II (thrombin) receptor-like 3
 71
 0.95
chr7_26192093_26192429 0.00 NFE2L3
 nuclear factor, erythroid 2-like 3
 401
 0.85
chr3_50358230_50358862 0.00 HYAL2
 hyaluronoglucosaminidase 2
 34
 0.93
chr16_77224093_77224603 0.00 MON1B
 MON1 secretory trafficking family member B
 384
 0.86
chr6_157801166_157801713 0.00 ZDHHC14
 zinc finger, DHHC-type containing 14
 726
 0.76
chr16_30662970_30663539 0.00 PRR14
 proline rich 14
 743
 0.43
chr8_56987192_56987600 0.00 CTA-397H3.3
245
 0.54
chr12_120632090_120632489 0.00 GCN1L1
 GCN1 general control of amino-acid synthesis 1-like 1 (yeast)
 224
 0.88
chr7_89783488_89783754 0.00 STEAP1
 six transmembrane epithelial antigen of the prostate 1
 68
 0.97
chr22_18893815_18894459 0.00 DGCR6
 DiGeorge syndrome critical region gene 6
 271
 0.91
chr1_43232974_43233556 0.00 C1orf50
 chromosome 1 open reading frame 50
 288
 0.65
chr14_23526484_23526858 0.00 CDH24
 cadherin 24, type 2
 76
 0.93

Histogram of CRE-TSS distances for CREs targeted by :

Histogram of

Rank distribution of CRE target scores:

Histogram of target CRE score.

Distribution of target CREs across different genomic regions:

Weighted Piechart of Weighted Piechart of Weighted Piechart of

Network of associatons between targets according to the STRING database.

StringDB image

First level regulatory network of HINFP1

PNG image of the network

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Gene Ontology Analysis

Gene overrepresentation in biological process category:

Log-likelihood per target  Total log-likelihood Term Description
0.1 0.3 GO:0035461 vitamin transmembrane transport(GO:0035461)
0.1 0.2 GO:0033622 integrin activation(GO:0033622)
0.0 0.1 GO:0051573 negative regulation of histone H3-K9 methylation(GO:0051573)
0.0 0.2 GO:0000301 retrograde transport, vesicle recycling within Golgi(GO:0000301)
0.0 0.1 GO:0050667 homocysteine metabolic process(GO:0050667)
0.0 0.1 GO:0016264 gap junction assembly(GO:0016264)
0.0 0.1 GO:0090231 regulation of spindle checkpoint(GO:0090231)
0.0 0.1 GO:0045897 regulation of transcription during mitosis(GO:0045896) positive regulation of transcription during mitosis(GO:0045897)
0.0 0.2 GO:0033169 histone H3-K9 demethylation(GO:0033169)
0.0 0.2 GO:0006516 glycoprotein catabolic process(GO:0006516)
0.0 0.2 GO:0001706 endoderm formation(GO:0001706)
0.0 0.1 GO:0060340 positive regulation of type I interferon-mediated signaling pathway(GO:0060340)
0.0 0.0 GO:0016115 diterpenoid catabolic process(GO:0016103) terpenoid catabolic process(GO:0016115) retinoic acid catabolic process(GO:0034653)
0.0 0.0 GO:0072367 regulation of lipid transport by regulation of transcription from RNA polymerase II promoter(GO:0072367)
0.0 0.1 GO:0007191 adenylate cyclase-activating dopamine receptor signaling pathway(GO:0007191)
0.0 0.1 GO:0045070 positive regulation of viral genome replication(GO:0045070)
0.0 0.1 GO:0009312 oligosaccharide biosynthetic process(GO:0009312)

Gene overrepresentation in cellular component category:

Log-likelihood per target  Total log-likelihood Term Description
0.0 0.1 GO:0032588 trans-Golgi network membrane(GO:0032588)

Gene overrepresentation in molecular function category:

Log-likelihood per target  Total log-likelihood Term Description
0.1 0.3 GO:0045118 azole transporter activity(GO:0045118) azole transmembrane transporter activity(GO:1901474)
0.0 0.2 GO:0008330 protein tyrosine/threonine phosphatase activity(GO:0008330)
0.0 0.1 GO:0008898 S-adenosylmethionine-homocysteine S-methyltransferase activity(GO:0008898)
0.0 0.1 GO:0000064 L-ornithine transmembrane transporter activity(GO:0000064)
0.0 0.1 GO:0031628 opioid receptor binding(GO:0031628)
0.0 0.1 GO:0000900 translation repressor activity, nucleic acid binding(GO:0000900)
0.0 0.3 GO:0005003 ephrin receptor activity(GO:0005003)
0.0 0.0 GO:0000978 RNA polymerase II core promoter proximal region sequence-specific DNA binding(GO:0000978) core promoter proximal region sequence-specific DNA binding(GO:0000987) core promoter proximal region DNA binding(GO:0001159)
0.0 0.1 GO:0005388 calcium-transporting ATPase activity(GO:0005388)
0.0 0.1 GO:0008499 UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase activity(GO:0008499)
0.0 0.0 GO:0008401 retinoic acid 4-hydroxylase activity(GO:0008401)
0.0 0.0 GO:0008934 inositol monophosphate 1-phosphatase activity(GO:0008934) inositol monophosphate phosphatase activity(GO:0052834)

Gene overrepresentation in curated gene sets: canonical pathways category:

Log-likelihood per target  Total log-likelihood Term Description
0.0 0.2 ST STAT3 PATHWAY STAT3 Pathway

Gene overrepresentation in curated gene sets: REACTOME pathways category:

Log-likelihood per target  Total log-likelihood Term Description
0.0 0.3 REACTOME SIGNAL REGULATORY PROTEIN SIRP FAMILY INTERACTIONS Genes involved in Signal regulatory protein (SIRP) family interactions